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hnf4α antibody  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology hnf4α antibody
    Hnf4α Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hnf4α antibody/product/Santa Cruz Biotechnology
    Average 90 stars, based on 1 article reviews
    hnf4α antibody - by Bioz Stars, 2026-02
    90/100 stars

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    Assessment of the diagnostic potential of HNF4α expression for detecting EGLs. (A) Accuracy of morphology‐based cytological analysis for detecting EGLs by smears. (B) Accuracy of HNF4α immunohistochemistry for detecting EGLs using FFPE issue specimens. (C) Accuracy of HNF4α immunocytochemistry for detecting EGLs by smears. AUC indicates area under the curve; EGL, endocervical glandular lesion; FFPE, formalin‐fixed paraffin‐embedded; HNF, hepatocyte nuclear factor; HNF4α, hepatocyte nuclear factor 4 alpha.

    Journal: Cancer Cytopathology

    Article Title: Hepatocyte nuclear factor 4 alpha immunocytochemistry: A useful marker for detecting endocervical glandular lesions in alcohol‐fixed smears

    doi: 10.1002/cncy.70034

    Figure Lengend Snippet: Assessment of the diagnostic potential of HNF4α expression for detecting EGLs. (A) Accuracy of morphology‐based cytological analysis for detecting EGLs by smears. (B) Accuracy of HNF4α immunohistochemistry for detecting EGLs using FFPE issue specimens. (C) Accuracy of HNF4α immunocytochemistry for detecting EGLs by smears. AUC indicates area under the curve; EGL, endocervical glandular lesion; FFPE, formalin‐fixed paraffin‐embedded; HNF, hepatocyte nuclear factor; HNF4α, hepatocyte nuclear factor 4 alpha.

    Article Snippet: Then, the slides were incubated with anti‐human HNF4α mouse monoclonal antibody (#H1415, recognizing the C‐terminal isoform, 1:1000; Perseus Proteomics, Tokyo, Japan) at 4°C overnight.

    Techniques: Diagnostic Assay, Expressing, Immunohistochemistry, Immunocytochemistry, Formalin-fixed Paraffin-Embedded

    Alcohol‐fixed cytological smears of EGLs and HNF4α immunocytochemical staining. (A,B) Case of cytologically diagnosed adenocarcinoma, histologically confirmed as HPVA ECA, showing a large cluster with nuclei with intense and diffuse HNF4α expression (arrowhead) and normal squamous cells without HNF4α staining (arrow). (C,D) Case of cytologically diagnosed AGC, histologically confirmed as NHPVA ECA, showing slightly nuclear atypia with HNF4α staining (arrowhead) and normal glands without HNF4α staining (arrow). (E,F) Case of cytologically diagnosed AGC, histologically confirmed as LEGH, showing yellowish mucin with HNF4α staining (arrowhead) and normal glands without HNF4α staining (arrow). (A,C,E) Papanicolaou staining, 200× magnification. (B,D,F) HNF4α immunocytochemical staining, 200× magnification. ECA indicates endocervical adenocarcinoma; EGL, endocervical glandular lesions; HNF, hepatocyte nuclear factor; HNF4α, hepatocyte nuclear factor 4 alpha; HPVA, human papillomavirus–associated; LEGH, lobular endocervical glandular hyperplasia; NHPVA, nonhuman papillomavirus–associated.

    Journal: Cancer Cytopathology

    Article Title: Hepatocyte nuclear factor 4 alpha immunocytochemistry: A useful marker for detecting endocervical glandular lesions in alcohol‐fixed smears

    doi: 10.1002/cncy.70034

    Figure Lengend Snippet: Alcohol‐fixed cytological smears of EGLs and HNF4α immunocytochemical staining. (A,B) Case of cytologically diagnosed adenocarcinoma, histologically confirmed as HPVA ECA, showing a large cluster with nuclei with intense and diffuse HNF4α expression (arrowhead) and normal squamous cells without HNF4α staining (arrow). (C,D) Case of cytologically diagnosed AGC, histologically confirmed as NHPVA ECA, showing slightly nuclear atypia with HNF4α staining (arrowhead) and normal glands without HNF4α staining (arrow). (E,F) Case of cytologically diagnosed AGC, histologically confirmed as LEGH, showing yellowish mucin with HNF4α staining (arrowhead) and normal glands without HNF4α staining (arrow). (A,C,E) Papanicolaou staining, 200× magnification. (B,D,F) HNF4α immunocytochemical staining, 200× magnification. ECA indicates endocervical adenocarcinoma; EGL, endocervical glandular lesions; HNF, hepatocyte nuclear factor; HNF4α, hepatocyte nuclear factor 4 alpha; HPVA, human papillomavirus–associated; LEGH, lobular endocervical glandular hyperplasia; NHPVA, nonhuman papillomavirus–associated.

    Article Snippet: Then, the slides were incubated with anti‐human HNF4α mouse monoclonal antibody (#H1415, recognizing the C‐terminal isoform, 1:1000; Perseus Proteomics, Tokyo, Japan) at 4°C overnight.

    Techniques: Staining, Expressing

    HNF4α immunocytochemical staining in smears of EGLs. (A,B) Cytologically and histologically diagnosed SCC lacking HNF4α expression. (C,D) Cytologically diagnosed ASC‐US and histologically confirmed as HPVA ECA showing a cluster with mild nuclear atypia with diffuse HNF4α expression on an inflammatory background. (A,C) Papanicolaou staining: (A) 200× and (C) 400× magnification. (B,D) HNF4α immunocytochemical staining (B) 200× and (D) 400× magnification. ASC‐US indicates atypical squamous cells of undetermined significance; ECA, endocervical adenocarcinoma; EGL, endocervical glandular lesion; HNF, hepatocyte nuclear factor; HNF4α, hepatocyte nuclear factor 4 alpha; HPVA, human papillomavirus–associated; SCC, squamous cell carcinoma.

    Journal: Cancer Cytopathology

    Article Title: Hepatocyte nuclear factor 4 alpha immunocytochemistry: A useful marker for detecting endocervical glandular lesions in alcohol‐fixed smears

    doi: 10.1002/cncy.70034

    Figure Lengend Snippet: HNF4α immunocytochemical staining in smears of EGLs. (A,B) Cytologically and histologically diagnosed SCC lacking HNF4α expression. (C,D) Cytologically diagnosed ASC‐US and histologically confirmed as HPVA ECA showing a cluster with mild nuclear atypia with diffuse HNF4α expression on an inflammatory background. (A,C) Papanicolaou staining: (A) 200× and (C) 400× magnification. (B,D) HNF4α immunocytochemical staining (B) 200× and (D) 400× magnification. ASC‐US indicates atypical squamous cells of undetermined significance; ECA, endocervical adenocarcinoma; EGL, endocervical glandular lesion; HNF, hepatocyte nuclear factor; HNF4α, hepatocyte nuclear factor 4 alpha; HPVA, human papillomavirus–associated; SCC, squamous cell carcinoma.

    Article Snippet: Then, the slides were incubated with anti‐human HNF4α mouse monoclonal antibody (#H1415, recognizing the C‐terminal isoform, 1:1000; Perseus Proteomics, Tokyo, Japan) at 4°C overnight.

    Techniques: Staining, Expressing

    HNF4α expression in paired FFPE surgical tissue specimens and alcohol‐fixed smears. (A,B) HPVA ECA in FFPE surgical tissue specimen with HNF4α expression in ECA glands (arrowhead) and without HNF4α expression in normal glands (arrow). (C,D) Paired alcohol‐fixed smear showing HNF4α expression in a large cluster consisting of atypical gland cells (arrowhead) and no HNF4α expression in nonatypical glands (arrow). (A) Hematoxylin–eosin staining, 100× magnification. (B) HNF4α immunohistochemical staining, 100× magnification. (C) Papanicolaou staining, 40× magnification. (D) HNF4α immunocytochemical staining, 40× magnification. ECA indicates endocervical adenocarcinoma; FFPE, formalin‐fixed paraffin‐embedded; HNF, hepatocyte nuclear factor; HNF4α, hepatocyte nuclear factor 4 alpha; HPVA, human papillomavirus–associated.

    Journal: Cancer Cytopathology

    Article Title: Hepatocyte nuclear factor 4 alpha immunocytochemistry: A useful marker for detecting endocervical glandular lesions in alcohol‐fixed smears

    doi: 10.1002/cncy.70034

    Figure Lengend Snippet: HNF4α expression in paired FFPE surgical tissue specimens and alcohol‐fixed smears. (A,B) HPVA ECA in FFPE surgical tissue specimen with HNF4α expression in ECA glands (arrowhead) and without HNF4α expression in normal glands (arrow). (C,D) Paired alcohol‐fixed smear showing HNF4α expression in a large cluster consisting of atypical gland cells (arrowhead) and no HNF4α expression in nonatypical glands (arrow). (A) Hematoxylin–eosin staining, 100× magnification. (B) HNF4α immunohistochemical staining, 100× magnification. (C) Papanicolaou staining, 40× magnification. (D) HNF4α immunocytochemical staining, 40× magnification. ECA indicates endocervical adenocarcinoma; FFPE, formalin‐fixed paraffin‐embedded; HNF, hepatocyte nuclear factor; HNF4α, hepatocyte nuclear factor 4 alpha; HPVA, human papillomavirus–associated.

    Article Snippet: Then, the slides were incubated with anti‐human HNF4α mouse monoclonal antibody (#H1415, recognizing the C‐terminal isoform, 1:1000; Perseus Proteomics, Tokyo, Japan) at 4°C overnight.

    Techniques: Expressing, Staining, Immunohistochemical staining, Formalin-fixed Paraffin-Embedded